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Characterization of prickle isoform-specific pk ^pk1 and pk ^sple1 mutations in Drosophila melanogaster.

ABSTRACT: We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk ^pk1 and pk ^sple1 mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5' UTR of pk ^pk1 or a premature stop codon in the second exon of pk ^sple1 . Additional likely benign missense mutations were identified in both mutant isoforms.

SUBMITTER: Lilienthal AJ

PROVIDER: S-EPMC9634456 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Characterization of prickle isoform-specific pk pk1 and pk sple1 mutations in Drosophila melanogaster.

Lilienthal Anthony J AJ Parida Mrutyunjaya M Manak J Robert JR

microPublication biology 20221020

We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk pk1 and pk sple1 mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5' UTR of pk pk1 or a premature stop codon in the second exon of pk sple1 . Additional likely benign ...[more]

PMID: 36338150

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Gene expression profiling of adult Drosophila melanogaster prickle-prickle mutant brains

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Prickle isoform participation in distinct polarization events in the Drosophila eye.

Project description:Planar cell polarity (PCP) signaling regulates several polarization events during development of ommatidia in the Drosophila eye, including directing chirality by polarizing a cell fate choice and determining the direction and extent of ommatidial rotation. The pksple isoform of the PCP protein Prickle is known to participate in the R3/R4 cell fate decision, but the control of other polarization events and the potential contributions of the three Pk isoforms have not been clarified. Here, by characterizing expression and subcellular localization of individual isoforms together with re-analyzing isoform specific phenotypes, we show that the R3/R4 fate decision, its coordination with rotation direction, and completion of rotation to a final ±90° rotation angle are separable polarization decisions with distinct Pk isoform requirements and contributions. Both pksple and pkpk can enforce robust R3/R4 fate decisions, but only pksple can correctly orient them along the dorsal-ventral axis. In contrast, pksple and pkpk can fully and interchangeably sustain coordination of rotation direction and rotation to completion. We propose that expression dynamics and competitive interactions determine isoform participation in these processes. We propose that the selective requirement for pksple to orient the R3/R4 decision and their interchangeability for coordination and completion of rotation reflects their previously described differential interaction with the Fat/Dachsous system which is known to be required for orientation of R3/R4 decisions but not for coordination or completion of rotation.

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Strabismus promotes recruitment and degradation of farnesylated prickle in Drosophila melanogaster planar polarity specification.

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