Dataset Information

A chromosome-level assembly of the widely used Rockefeller strain of Aedes aegypti, the yellow fever mosquito.

ABSTRACT: Aedes aegypti is the vector of important human diseases, and genomic resources are crucial in facilitating the study of A. aegypti and its ecosystem interactions. Several laboratory-acclimated strains of this mosquito have been established, but the most used strain in toxicology studies is "Rockefeller," which was originally collected and established in Cuba 130 years ago. A full-length genome assembly of another reference strain, "Liverpool," was published in 2018 and is the reference genome for the species (AaegL5). However, genetic studies with the Rockefeller strain are complicated by the availability of only the Liverpool strain as the reference genome. Differences between Liverpool and Rockefeller have been known for decades, particularly in the expression of genes relevant to mosquito behavior and vector control (e.g. olfactory). These differences indicate that AaegL5 is likely not fully representative of the Rockefeller genome, presenting potential impediments to research. Here, we present a chromosomal-level assembly and annotation of the Rockefeller genome and a comparative characterization vs the Liverpool genome. Our results set the stage for a pan-genomic approach to understanding evolution and diversity within this important disease vector.

SUBMITTER: Fisher CR

PROVIDER: S-EPMC9635639 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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A chromosome-level assembly of the widely used Rockefeller strain of Aedes aegypti, the yellow fever mosquito.

Fisher Cera R CR Wilson Michael M Scott Jeffrey G JG

G3 (Bethesda, Md.) 20221101 11

Aedes aegypti is the vector of important human diseases, and genomic resources are crucial in facilitating the study of A. aegypti and its ecosystem interactions. Several laboratory-acclimated strains of this mosquito have been established, but the most used strain in toxicology studies is "Rockefeller," which was originally collected and established in Cuba 130 years ago. A full-length genome assembly of another reference strain, "Liverpool," was published in 2018 and is the reference genome fo ...[more]

PMID: 36086997

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Project description:BackgroundAedes aegypti, the yellow fever mosquito, is an efficient vector of arboviruses and a convenient model system for laboratory research. Extensive linkage mapping of morphological and molecular markers localized a number of quantitative trait loci (QTLs) related to the mosquito's ability to transmit various pathogens. However, linking the QTLs to Ae. aegypti chromosomes and genomic sequences has been challenging because of the poor quality of polytene chromosomes and the highly fragmented genome assembly for this species.Methodology/principal findingsBased on the approach developed in our previous study, we constructed idiograms for mitotic chromosomes of Ae. aegypti based on their banding patterns at early metaphase. These idiograms represent the first cytogenetic map developed for mitotic chromosomes of Ae. aegypti. One hundred bacterial artificial chromosome clones carrying major genetic markers were hybridized to the chromosomes using fluorescent in situ hybridization. As a result, QTLs related to the transmission of the filarioid nematode Brugia malayi, the avian malaria parasite Plasmodium gallinaceum, and the dengue virus, as well as sex determination locus and 183 Mbp of genomic sequences were anchored to the exact positions on Ae. aegypti chromosomes. A linear regression analysis demonstrated a good correlation between positions of the markers on the physical and linkage maps. As a result of the recombination rate variation along the chromosomes, 12 QTLs on the linkage map were combined into five major clusters of QTLs on the chromosome map.ConclusionThis study developed an integrated linkage, chromosome, and genome map-iMap-for the yellow fever mosquito. Our discovery of the localization of multiple QTLs in a few major chromosome clusters suggests a possibility that the transmission of various pathogens is controlled by the same genomic loci. Thus, the iMap will facilitate the identification of genomic determinants of traits responsible for susceptibility or refractoriness of the mosquito to diverse pathogens.

Dataset Information

A chromosome-level assembly of the widely used Rockefeller strain of Aedes aegypti, the yellow fever mosquito.

Publications

A chromosome-level assembly of the widely used Rockefeller strain of Aedes aegypti, the yellow fever mosquito.

Similar Datasets

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