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Stepwise molecular mechanisms responsible for chemoresistance in bladder cancer cells.


ABSTRACT: Chemotherapy resistance is an obstacle to cancer therapy and is considered a major cause of recurrence. Thus, understanding the mechanisms of chemoresistance is critical to improving the prognosis of patients. Here, we have established a stepwise gemcitabine-resistant T24 bladder cancer cell line to understand the molecular mechanisms of chemoresistance within cancer cells. The characteristics of the stepwise chemoresistance cell line were divided into 4 phases (parental, early, intermediate, and late phases). These four phase cells showed increasingly aggressive phenotypes in vitro and in vivo experiments with increasing phases and revealed the molecular properties of the biological process from parent cells to phased gemcitabine-resistant cell line (GRC). Taken together, through the analysis of gene expression profile data, we have characterized gene set of each phase indicating the response to anticancer drug treatment. Specifically, we identified a multigene signature (23 genes including GATA3, APOBEC3G, NT5E, MYC, STC1, FOXD1, SMAD9) and developed a chemoresistance score consisting of that could predict eventual responsiveness to gemcitabine treatment. Our data will contribute to predicting chemoresistance and improving the prognosis of bladder cancer patients.

SUBMITTER: Mun JY 

PROVIDER: S-EPMC9640638 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Stepwise molecular mechanisms responsible for chemoresistance in bladder cancer cells.

Mun Jeong-Yeon JY   Baek Seung-Woo SW   Jeong Mi-So MS   Jang In-Hwan IH   Lee Se-Ra SR   You Jae-Young JY   Kim Jeong-Ah JA   Yang Gi-Eun GE   Choi Yung-Hyun YH   Kim Tae-Nam TN   Chu In-Sun IS   Leem Sun-Hee SH  

Cell death discovery 20221107 1


Chemotherapy resistance is an obstacle to cancer therapy and is considered a major cause of recurrence. Thus, understanding the mechanisms of chemoresistance is critical to improving the prognosis of patients. Here, we have established a stepwise gemcitabine-resistant T24 bladder cancer cell line to understand the molecular mechanisms of chemoresistance within cancer cells. The characteristics of the stepwise chemoresistance cell line were divided into 4 phases (parental, early, intermediate, an  ...[more]

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