RAGE displays sex-specific differences in obesity-induced adipose tissue insulin resistance.

ABSTRACT: The receptor for advanced glycation end products (RAGE) plays an important role in obesity-associated insulin sensitivity. We have also previously reported that RAGE deficiency improved insulin resistance in obesity-induced adipose tissue. The current study was aimed to elucidate the sex-specific mechanism of RAGE deficiency in adipose tissue metabolic regulation and systemic glucose homeostasis. RAGE-deficient (RAGE-/-) mice were fed a high-fat diet (HFD) and subjected to glucose and insulin tolerance tests. Subcutaneous adipose tissue (sAT) was collected, and macrophage polarization was assessed by quantitative real-time PCR. Immunoblotting was performed to evaluate the insulin signaling in adipose tissues. Under HFD feeding conditions, body weight and adipocyte size of female RAGE defic

SUBMITTER: Du Z 

PROVIDER: S-EPMC9641909 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature