TurboID functions as an efficient biotin ligase for BioID applications in Xenopus embryos.

ABSTRACT: BioID is a proximity labeling strategy whose goal is to identify in vivo protein-protein interactions. The central components of this strategy are modified biotin ligase enzymes that promiscuously add biotin groups to proteins in close proximity. The transferred biotin group provides a powerful tag for purification and thus identification of interacting proteins. While a variety of modified biotin ligases were created for BioID, the original enzymes were inefficient, required long incubation times, and high intracellular biotin concentrations for protein labeling. These limitations hinder the application of BioID in contexts such as developing embryos where processes such as cell division and cell fate decisions occur rapidly. Recently, a new biotin ligase called TurboID was developed that

SUBMITTER: Kanzler CR 

PROVIDER: S-EPMC9643680 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature