Production and statistical optimization of cholesterol-oxidase generated by Streptomyces sp. AN strain.

ABSTRACT:

Background

Cholesterol oxidases (CHOs) have attracted enormous attention because of their wide biotechnological potential. The present study explores the production of CHOs by Streptomyces sp. AN. Evaluation of culture conditions affecting enzyme production, medium optimization and released metabolite characteristics were also investigated.

Results

The current work reports the isolation of 37 colonies (bacteria/actinobacteria) with different morphotypes from different soil/water samples. The isolate-coded AN was selected for its high potency for CHO production. Morphological characteristics and the obtained partial sequence of 16srRNA of AN showed 99.38% identity to Streptomyces sp. strain P12-37. Factors affecting CHO production were evaluated using Plackett-Burman (PB) and

SUBMITTER: Alam AA 

PROVIDER: S-EPMC9649583 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature