Single-cell transcriptomics reveals cellular heterogeneity and molecular stratification of cervical cancer.

ABSTRACT: Cervical cancer (CC) is the most common gynecological malignancy, whose cellular heterogeneity has not been fully understood. Here, we performed single-cell RNA sequencing (scRNA-seq) to survey the transcriptomes of 57,669 cells derived from three CC tumors with paired normal adjacent non-tumor (NAT) samples. Single-cell transcriptomics analysis revealed extensive heterogeneity in malignant cells of human CCs, wherein epithelial subpopulation exhibited different genomic and transcriptomic signatures. We also identified cancer-associated fibroblasts (CAFs) that may promote tumor progression of CC, and further distinguished inflammatory CAF (iCAF) and myofibroblastic CAF (myCAF). CD8⁺ T cell diversity revealed both proliferative (MKI67⁺) and non-cycling exhausted (PDCD1

SUBMITTER: Li C 

PROVIDER: S-EPMC9649750 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature