Controlling Nuclease Degradation of Wireframe DNA Origami with Minor Groove Binders.

ABSTRACT: Viruslike particles (VLPs) fabricated using wireframe DNA origami are emerging as promising vaccine and gene therapeutic delivery platforms due to their programmable nature that offers independent control over their size and shape, as well as their site-specific functionalization. As materials that biodegrade in the presence of endonucleases, specifically DNase I and II, their utility for the targeting of cells, tissues, and organs depends on their stability in vivo. Here, we explore minor groove binders (MGBs) as specific endonuclease inhibitors to control the degradation half-life of wireframe DNA origami. Bare, unprotected DNA-VLPs composed of two-helix edges were found to be stable in fetal bovine serum under typical cell culture conditions and in human serum for 24 h but degraded with

SUBMITTER: Wamhoff EC 

PROVIDER: S-EPMC9649841 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature