Identification of protein profile in metacyclic and amastigote-like stages of Leishmania tropica: a proteomic approach.

ABSTRACT: Leishmaniasis is a tropical disease that leads to various clinical phenotypes. This study aimed to investigate protein expression changes in metacyclic and amastigote-like stages of L. tropica isolated from Iranian cutaneous leishmaniasis patients. Isolated samples were cultured and species type identified using PCR-RFLP technique. The promastigotes were grown in RPMI1640 media and differentiated to metacyclic and amastigote-like forms, followed by the extracted proteins of both successive stages carried out for proteomics and bioinformatics analysis. Using SWATH-MS quantitative proteomics technique, a total 176 and 155 distinct proteins were identified in metacyclic and axenic amastigote stages, respectively. Of these, 65 proteins were altered significantly (p-value < 0.05 and fold change

SUBMITTER: Ashrafmansouri M 

PROVIDER: S-EPMC9653527 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature