TBX20 Improves Contractility and Mitochondrial Function During Direct Human Cardiac Reprogramming.

ABSTRACT:

Background

Direct cardiac reprogramming of fibroblasts into cardiomyocytes has emerged as a promising strategy to remuscularize injured myocardium. However, it is insufficient to generate functional induced cardiomyocytes from human fibroblasts using conventional reprogramming cocktails, and the underlying molecular mechanisms are not well studied.

Methods

To discover potential missing factors for human direct reprogramming, we performed transcriptomic comparison between human induced cardiomyocytes and functional cardiomyocytes.

Results

We identified TBX20 (T-box transcription factor 20) as the top cardiac gene that is unable to be activated by the MGT133 reprogramming cocktail (MEF2C, GATA4, TBX5, and miR-133). TBX20 is required fo

SUBMITTER: Tang Y 

PROVIDER: S-EPMC9662826 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature