An Optimized Protocol for Human Direct Cardiac Reprogramming.
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ABSTRACT:
SUBMITTER: Garbutt TA
PROVIDER: S-EPMC7390466 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
An Optimized Protocol for Human Direct Cardiac Reprogramming.
Garbutt Tiffany A TA Zhou Yang Y Zhou Yang Y Keepers Benjamin B Liu Jiandong J Qian Li L
STAR protocols 20200603 1
Direct cardiac reprogramming, the conversion of fibroblasts into cardiomyocyte-like cells (iCMs), is an attractive approach to heal the injured heart. Here we present a new approach to human cardiac reprogramming that utilizes a polycistronic three-factor reprogramming cocktail and one microRNA. Our protocol produces cardiac Troponin T positive human iCMs (hiCMs) at an efficiency of 40%-60%, approximately double that of previous protocols, within just 2 weeks. The resulting hiCMs display cardiom ...[more]
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