Three-dimensional imaging and analysis of pathological tissue samples with de novo generation of citrate-based fluorophores.

ABSTRACT: Two-dimensional (2D) histopathology based on the observation of thin tissue slides is the current paradigm in diagnosis and prognosis. However, labeling strategies in conventional histopathology are limited in compatibility with 3D imaging combined with tissue clearing techniques. Here, we present a rapid and efficient volumetric imaging technique of pathological tissues called 3D tissue imaging through de novo formation of fluorophores, or 3DNFC, which is the integration of citrate-based fluorogenic reaction DNFC and tissue clearing techniques. 3DNFC markedly increases the fluorescence intensity of tissues by generating fluorophores on nonfluorescent amino-terminal cysteine and visualizes the 3D structure of the tissues to provide their anatomical morphology and volumetric information. Fu

SUBMITTER: Pac J 

PROVIDER: S-EPMC9668299 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature