Dataset Information

Investigation of Photocatalyst Composites for Pollutant Degradation in a Microslit Reactor Utilizing High Throughput Screening Techniques.

ABSTRACT: The high-throughput screening investigations on TiO₂ based photocatalyst composites presented here have been carried out in a 60-fold parallel photoreactor. Additional catalyst testing was performed in a microslit reactor system with immobilized catalysts. For further enhancing the photocatalytic activity of TiO₂ (P25), composites of P25 and, for example, Bi₂ O₃ , CeO₂ , g-C₃ N₄ , WO₃ or ZnO were formulated in different nominal molar ratios. The catalysts' performances were assessed by their conversion of 17α-ethinyl estradiol (EE2) in aqueous solutions, determined by LC-MS. Findings show rapid EE2 conversions in short residence times. The extensive testing of catalysts led to the conclusion that the photocatalytic conversion is rather a function of residence time than a function of the materials utilized. This makes adequate process development seem more important than material development. The novelty of this contribution lies in the unique combination of testing a wide range of composite catalysts in a unique microreactor geometry.

SUBMITTER: Engelhardt TB

PROVIDER: S-EPMC9668610 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Investigation of Photocatalyst Composites for Pollutant Degradation in a Microslit Reactor Utilizing High Throughput Screening Techniques.

Engelhardt Tony B TB Schmitz-Stöwe Sabine S Schwarz Thomas T Stöwe Klaus K

ChemistryOpen 20221101 11

The high-throughput screening investigations on TiO2 based photocatalyst composites presented here have been carried out in a 60-fold parallel photoreactor. Additional catalyst testing was performed in a microslit reactor system with immobilized catalysts. For further enhancing the photocatalytic activity of TiO2 (P25), composites of P25 and, for example, Bi2 O3 , CeO2 , g-C3 N4 , WO3 or ZnO were formulated i ...[more]

PMID: 36385481

Similar Datasets

Project description:BACKGROUND:The presence of a wide range of bioactive organic pollutants in wastewater and municipal water sources is raising concerns about their potential effects on humans. Not surprisingly, various approaches are being explored that can efficiently degrade these persistent organic pollutants. Use of peroxidases has recently been recognized as a novel remediation approach that may have potential advantages over conventional degradation techniques. However, testing the abilities of different peroxidases to degrade diverse emerging pollutants is tedious and cumbersome. RESULTS:In the present study, we present a rapid and robust approach to easily test the degradability of 21 different emerging pollutants by five different peroxidases (soybean peroxidase, chloroperoxidase, lactoperoxidase, manganese peroxidase, and horseradish peroxidase) using an LC-MSMS approach. Furthermore, this approach was also used to examine the role of a redox mediator in these enzymatic degradation assays. Our results show that some of the organic pollutants can be easily degraded by all five of the peroxidases tested, whereas others are only degraded by a specific peroxidase (or when a redox mediator was present) and there are some that are completely resistant to degradation by any of the peroxidases tested (even in the presence of a redox mediator). The degradation of furosemide and trimethoprim by soybean peroxidase and chloroperoxidase, respectively, was investigated in detail by examining the transformation products generated during their degradation. Some of the products generated during enzymatic breakdown of these pollutants have been previously reported by others, however, we report many new transformation products. CONCLUSIONS:LC-MSMS approaches, like the one described here, can be used to rapidly evaluate the potential of different peroxidases (and redox requirements) to be used as bioremediation agents. Our preliminary result shows peroxidases hold tremendous potential for being used in a final wastewater treatment step.

Project description:Pregnane X receptor (PXR) is a xenobiotic-sensing nuclear receptor with a well-established role in regulating drug metabolism and clearance. Recent studies have shown that PXR is involved in cell proliferation, apoptosis, immune response, and energy homeostasis. It is important to identify compounds that may modulate PXR activity to prevent drug-drug interactions, distinguish chemicals which could potentially generate toxicity, and identify compounds for further development towards therapeutic usage. In this study, we have screened the National Center for Advancing Translational Sciences (NCATS) Pharmacologically Active Chemical Toolbox (NPACT) library, which consists of 5,099 unique pharmacologically active synthetic and naturally derived small molecules to identify PXR antagonists. Ninety-four compounds were identified as potential PXR antagonists through a primary screen and 66 were confirmed in a confirmation study. Of these compounds, twenty potential PXR antagonists, including gamma-secretase modulator 2 (GSM2) and fusidic acid, were selected for further study based on their efficacy, potency, and novelty. Their PXR inhibition abilities were assessed by examining their effects on cytrochrome P450 (CYP) 3A4 mRNA expression using metabolically competent HepaRG cells. Additionally, a pharmacological inhibition assay using various concentrations of rifampicin as a stimulator was performed in HepG2-CYP3A4-hPXR cells to confirm the activity of the 20 selected compounds against PXR. Finally, HepaRG cells were used to confirm PXR antagonism by verification of a concentration-dependent decrease of CYP3A4 when co-treated with the known PXR agonist, rifampicin. Additionally, the potent actives were further investigated using molecular docking to find the potential interactions of the novel ligands with the active sites of hPXR. To our knowledge from the current study, GSM2 and fusidic acid have been identified as novel PXR antagonists, which provides useful information for further investigation regarding possible drug-drug interactions, as well as the detection of potential therapeutic effects or other toxic consequences.

Project description:Bacterial and organic pollutants are major problems with potential adverse impacts on human health and the environment. A promising strategy to alleviate these impacts consists in designing innovative photocatalysts with a wider spectrum of application. In this paper, we report the improved photocatalytic and antibacterial activities of chemically precipitated Ag3PO4 microcrystals by the incorporation of W at doping levels 0.5, 1, and 2 mol %. The presence of W directly influences the crystallization of Ag3PO4, affecting the morphology, particle size, and surface area of the microcrystals. Also, the characterization via experimental and theoretical approaches evidenced a high density of disordered [AgO4], [PO4], and [WO4] structural clusters due to the substitution of P5+ by W6+ into the Ag3PO4 lattice. This leads to new defect-related energy states, which decreases the band gap energy of the materials (from 2.27 to 2.04 eV) and delays the recombination of e'-h• pairs, leading to an enhanced degradation process. As a result of such behaviors, W-doped Ag3PO4 (Ag3PO4:W) is a better visible-light photocatalyst than Ag3PO4, demonstrated here by the photodegradation of potential environmental pollutants. The degradation of rhodamine B dye was 100% in 4 min for Ag3PO4:W 1%, and for Ag3PO4, the obtained result was 90% of degradation in 15 min of reaction. Ag3PO4:W 1% allowed the total degradation of cephalexin antibiotic in only 4 min, whereas pure Ag3PO4 took 20 min to achieve the same result. For the degradation of imidacloprid insecticide, Ag3PO4:W 1% allowed 90% of degradation, whereas Ag3PO4 allowed 40%, both in 20 min of reaction. Moreover, the presence of W-dopant results in a 16-fold improvement of bactericidal performance against methicillin-resistant Staphylococcus aureus. The outstanding results using the Ag3PO4:W material demonstrated its potential multifunctionality for the control of organic pollutants and bacteria in environmental applications.

Dataset Information

Investigation of Photocatalyst Composites for Pollutant Degradation in a Microslit Reactor Utilizing High Throughput Screening Techniques.

Publications

Investigation of Photocatalyst Composites for Pollutant Degradation in a Microslit Reactor Utilizing High Throughput Screening Techniques.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets