Dataset Information

Retrospective molecular investigation of Mayaro and Oropouche viruses at the human-animal interface in West-central Brazil, 2016-2018.

ABSTRACT: Mayaro virus (MAYV, Togaviridae) and Oropouche orthobunyavirus (OROV, Peribunyaviridae) are emerging enzootic arboviruses in Latin America. Outbreaks of febrile illness associated with MAYV and OROV have been reported among humans mainly in the northern region of Brazil since the 1980s, and recent data suggest these viruses have circulated also in more populated areas of western Brazil. MAYV shares mosquito vectors with yellow fever virus and it has been historically detected during yellow fever epidemics. Aiming to investigate the transmission of OROV and MAYV at the human-animal interface during a yellow fever, chikungunya and Zika outbreaks in Brazil, we conducted a retrospective molecular investigation in 810 wild and domestic animals, 106 febrile patients, and 22.931 vectors collected from 2016 to 2018 in Cuiaba and Campo Grande metropolitan regions, western Brazil. All samples tested negative for OROV and MAYV RNA by RT-qPCR. Findings presented here suggest no active circulation of MAYV and OROV in the sampled hosts. Active surveillance and retrospective investigations are instrumental approaches for the detection of cryptic and subclinical activity of enzootic arboviruses and together serve as a warning system to implement appropriate actions to prevent outbreaks.

SUBMITTER: Dias HG

PROVIDER: S-EPMC9671456 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Retrospective molecular investigation of Mayaro and Oropouche viruses at the human-animal interface in West-central Brazil, 2016-2018.

Dias Helver Gonçalves HG de Lima Raquel Curtinhas RC Barbosa Luciana Santos LS Souza Thiara Manuele Alves de TMA Badolato-Correa Jessica J Maia Laura Marina Siqueira LMS Ferreira Raquel da Silva RDS Neves Nilvanei Aparecido da Silva NADS Costa Michell Charlles de Souza MCS Martins Leticia Ramos LR Souza Emerson Marques de EM Carvalho Michellen Dos Santos MDS Araujo-Oliveira Alexandre de A Marques William de Almeida WA Sabino-Santos Gilberto G Marques Marcio Schafer MS Macedo Gabriel Carvalho de GC Nantes Wesley Arruda Gimenes WAG Santos Filipe Martins FM Santos Filipe Martins FM Netto Claudia Coutinho CC Morgado Thais Oliveira TO Bianchini Mateus de Assis MA Correa Sandra Helena Ramiro SHR Almeida Júlia Ramos de JR Campos Larissa Pratta LP Souza Isabelle Marino de IM Barreto Wanessa Teixeira Gomes WTG Porfírio Grasiela G Alencar Jeronimo Augusto Fonseca JAF Herrera Heitor Miraglia HM Shlessarenko Renata Dezengrini RD Cunha Rivaldo Venancio da RVD Azeredo Elzinandes Leal de EL Salyer Stephanie J SJ Komar Nicholas N Pauvolid-Corrêa Alex A Dos Santos Flávia Barreto FB

PloS one 20221117 11

Mayaro virus (MAYV, Togaviridae) and Oropouche orthobunyavirus (OROV, Peribunyaviridae) are emerging enzootic arboviruses in Latin America. Outbreaks of febrile illness associated with MAYV and OROV have been reported among humans mainly in the northern region of Brazil since the 1980s, and recent data suggest these viruses have circulated also in more populated areas of western Brazil. MAYV shares mosquito vectors with yellow fever virus and it has been historically detected during yellow fever ...[more]

PMID: 36395285

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Project description:Zika virus (ZIKV) was first discovered in 1947 in Uganda but was not considered a public health threat until 2007 when it found to be the source of epidemic activity in Asia. Epidemic activity spread to Brazil in 2014 and continued to spread throughout the tropical and subtropical regions of the Americas. Despite ZIKV being zoonotic in origin, information about transmission, or even exposure of non-human vertebrates and mosquitoes to ZIKV in the Americas, is lacking. Accordingly, from February 2017 to March 2018, we sought evidence of sylvatic ZIKV transmission by sampling whole blood from approximately 2000 domestic and wild vertebrates of over 100 species in West-Central Brazil within the active human ZIKV transmission area. In addition, we collected over 24,300 mosquitoes of at least 17 genera and 62 species. We screened whole blood samples and mosquito pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase chain reaction (RT-PCR) in a SYBR Green platform. Positives were confirmed using ZIKV-specific envelope gene real-time RT-PCR and nucleotide sequencing. Of the 2068 vertebrates tested, none were ZIKV positive. Of the 23,315 non-engorged mosquitoes consolidated into 1503 pools tested, 22 (1.5%) with full data available showed some degree of homology to insect-specific flaviviruses. To identify previous exposure to ZIKV, 1498 plasma samples representing 62 species of domestic and sylvatic vertebrates were tested for ZIKV-neutralizing antibodies by plaque reduction neutralization test (PRNT90). From these, 23 (1.5%) of seven species were seropositive for ZIKV and negative for dengue virus serotype 2, yellow fever virus, and West Nile virus, suggesting potential monotypic reaction for ZIKV. Results presented here suggest no active transmission of ZIKV in non-human vertebrate populations or in alternative vector candidates, but suggest that vertebrates around human populations have indeed been exposed to ZIKV in West-Central Brazil.

Dataset Information

Retrospective molecular investigation of Mayaro and Oropouche viruses at the human-animal interface in West-central Brazil, 2016-2018.

Publications

Retrospective molecular investigation of Mayaro and Oropouche viruses at the human-animal interface in West-central Brazil, 2016-2018.

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