Testis electroporation coupled with autophagy inhibitor to treat non-obstructive azoospermia.

ABSTRACT: Infertility affects 10%-20% of the population in most countries, with approximately half of those cases resulting from male infertility. Although millions of infertile men are able to have children with the assistance of reproductive technology, individuals with non-obstructive azoospermia (NOA) syndrome are unable to do so because they lack functional sperm. Therefore, some other strategies for infertile NOA men are still urgently needed. Our current study uses an NOA-like mouse model to optimize microinjection and a subsequent electroporation method to test potential treatment strategies. We showed that the spermatogenetic process could be partially rescued in young Stra8-Rnf20 ^-/- mice with microinjection and subsequent electroporation of Rnf20 plasmids into the

SUBMITTER: Wang L 

PROVIDER: S-EPMC9672891 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature