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Altered fecal microbiome and metabolome in adult patients with non-cystic fibrosis bronchiectasis.


ABSTRACT:

Background

Emerging experimental and epidemiological evidence highlights a crucial cross-talk between the intestinal flora and the lungs, termed the "gut-lung axis". However, the function of the gut microbiota in bronchiectasis remains undefined. In this study, we aimed to perform a multi-omics-based approach to identify the gut microbiome and metabolic profiles in patients with bronchiectasis.

Methods

Fecal samples collected from non-CF bronchiectasis patients (BE group, n = 61) and healthy volunteers (HC group, n = 37) were analyzed by 16 S ribosomal RNA (rRNA) sequencing. The BE group was divided into two groups based on their clinical status: acute exacerbation (AE group, n = 31) and stable phase (SP group, n = 30). Further, metabolome (lipid chromatography-mass spectrometry, LC-MS) analyses were conducted in randomly selected patients (n = 29) and healthy volunteers (n = 31).

Results

Decreased fecal microbial diversity and differential microbial and metabolic compositions were observed in bronchiectasis patients. Correlation analyses indicated associations between the differential genera and clinical parameters such as bronchiectasis severity index (BSI). Disease-associated gut microbiota was screened out, with eight genera exhibited high accuracy in distinguishing SP patients from HCs in the discovery cohort and validation cohort using a random forest model. Further correlation networks were applied to illustrate the relations connecting disease-associated genera and metabolites.

Conclusion

The study uncovered the relationships among the decreased fecal microbial diversity, differential microbial and metabolic compositions in bronchiectasis patients by performing a multi-omics-based approach. It is the first study to characterize the gut microbiome and metabolome in bronchiectasis, and to uncover the gut microbiota's potentiality as biomarkers for bronchiectasis.

Trial registration

 This study is registered with ClinicalTrials.gov, number NCT04490447.

SUBMITTER: Wang WW 

PROVIDER: S-EPMC9675243 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Publications

Altered fecal microbiome and metabolome in adult patients with non-cystic fibrosis bronchiectasis.

Wang Wen-Wen WW   Mao Bei B   Liu Yang Y   Gu Shu-Yi SY   Lu Hai-Wen HW   Bai Jiu-Wu JW   Liang Shuo S   Yang Jia-Wei JW   Li Jian-Xiong JX   Su Xiao X   Hu Hai-Yang HY   Wang Chen C   Xu Jin-Fu JF  

Respiratory research 20221119 1


<h4>Background</h4>Emerging experimental and epidemiological evidence highlights a crucial cross-talk between the intestinal flora and the lungs, termed the "gut-lung axis". However, the function of the gut microbiota in bronchiectasis remains undefined. In this study, we aimed to perform a multi-omics-based approach to identify the gut microbiome and metabolic profiles in patients with bronchiectasis.<h4>Methods</h4>Fecal samples collected from non-CF bronchiectasis patients (BE group, n = 61)  ...[more]

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