Investigation of reversible histone acetylation and dynamics in gene expression regulation using 3D liver spheroid model.

ABSTRACT:

Background

Three-dimensional (3D) cell culture has emerged as an alternative approach to 2D flat culture to model more accurately the phenotype of solid tissue in laboratories. Culturing cells in 3D more precisely recapitulates physiological conditions of tissues, as these cells reduce activities related to proliferation, focusing their energy consumption toward metabolism and homeostasis.

Results

Here, we demonstrate that 3D liver spheroids are a suitable system to model chromatin dynamics and response to epigenetics inhibitors. To delay necrotic tissue formation despite proliferation arrest, we utilize rotating bioreactors that apply active media diffusion and low shearing forces. We demonstrate that the proteome and the metabolome of our model resemble typical liver funct

SUBMITTER: Stransky S 

PROVIDER: S-EPMC9677638 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature