Molecular diagnosis of rhino-orbital mucormycosis in a COVID-19 setting.

ABSTRACT:

Purpose

Mucormycosis is a severe fungal infection caused by species of the order Mucorales. Early and accurate diagnosis is a prerequisite in the management of the disease. In the present study, we evaluated and compared two PCR-based techniques for the diagnosis and identification of mucormycosis in patients with rhino-orbital mucormycosis (ROM) post-COVID-19.

Methods

Diagnosed clinically and radiologically, 25 patients of ROM were included in the study and endoscopically or blind collected nasal swabs or orbital tissues were submitted for microbiological evaluation (direct microscopy + culture) and PCR using primers targeting two different loci (ITS and 28S rDNA region) for diagnosis. All PCR products were further processed for species identification using Sanger sequencin

SUBMITTER: Khapuinamai A 

PROVIDER: S-EPMC9684940 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature