Deep Proteome Profiling with Reduced Carryover Using Superficially Porous Microfabricated nanoLC Columns.

ABSTRACT: In the field of liquid chromatography-mass spectrometry (LC-MS)-based proteomics, increases in the sampling depth and proteome coverage have mainly been accomplished by rapid advances in mass spectrometer technology. The comprehensiveness and quality of the data that can be generated do, however, also depend on the performance provided by nano-liquid chromatography (nanoLC) separations. Proper selection of reversed-phase separation columns can be important to provide the MS instrument with peptides at the highest possible concentration and separated at the highest possible resolution. In the current contribution, we evaluate the use of the prototype generation 2 μPAC nanoLC columns, which use C18-functionalized superficially porous micropillars as a stationary phase. When compared to tradi

SUBMITTER: Stejskal K 

PROVIDER: S-EPMC9685595 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature