Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits.

ABSTRACT:

Background

To evaluate tissue regeneration of the urinary bladder after the implantation of a decellularized vein sown with autologous adipose-derived mesenchymal stem cells (ASC) on luminal surfaces.

Methods

New Zealand rabbits (n = 10) were distributed in two groups: Group Bioscaffold alone (G1)-decellularized vena cava (1 cm²) was implanted, and Group Bioscaffold plus ACSs (G2)-decellularized vena cava (1 cm²) containing ASCs were implanted. ASCs were expanded, characterized, and maintained for one week in culture with a decellularized vein scaffold. The implants were performed under general anesthesia using a continuous suture pattern. Afterward, 21 d (day) specimens were collected and analyzed by hematoxylin and eosin (HE) histology and scan

SUBMITTER: Piovesana TR 

PROVIDER: S-EPMC9687924 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature