Dataset Information

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits.

ABSTRACT:

Background

To evaluate tissue regeneration of the urinary bladder after the implantation of a decellularized vein sown with autologous adipose-derived mesenchymal stem cells (ASC) on luminal surfaces.

Methods

New Zealand rabbits (n = 10) were distributed in two groups: Group Bioscaffold alone (G1)-decellularized vena cava (1 cm²) was implanted, and Group Bioscaffold plus ACSs (G2)-decellularized vena cava (1 cm²) containing ASCs were implanted. ASCs were expanded, characterized, and maintained for one week in culture with a decellularized vein scaffold. The implants were performed under general anesthesia using a continuous suture pattern. Afterward, 21 d (day) specimens were collected and analyzed by hematoxylin and eosin (HE) histology and scanning electron microscopy (SEM).

Results

The integrity of the urinary bladder was maintained in both groups. A superior regenerative process was observed in the G2 group, compared to the G1 group. We observed a greater urothelial epithelialization and maturity of the mucosa and submucosa fibroblasts. Furthermore, SEM demonstrated a notable amount of urothelial villus in the G2 group.

Conclusion

Decellularized vena cava scaffolds were able to maintain the integrity of the urinary bladder in the proposed model. In addition, ASCs accelerated the regenerative process development, observed primarily by the new urothelial epithelization and the maturity of mucosa and submucosa fibroblasts.

SUBMITTER: Piovesana TR

PROVIDER: S-EPMC9687924 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Publications

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits.

Piovesana Tadeu Ravazi TR Rodrigues Lenize da Silva LDS Bovolato Ana Livia de Carvalho ALC Rodríguez-Sánchez Diego Noé DN Rinaldi Jaqueline Carvalho JC Santos Nilton José NJ Mori Julia Calvi JC Lourenção Pedro Luiz Toledo de Arruda PLTA Birch Lynn L Bertanha Matheus M

Biomedicines 20221104 11

<h4>Background</h4>To evaluate tissue regeneration of the urinary bladder after the implantation of a decellularized vein sown with autologous adipose-derived mesenchymal stem cells (ASC) on luminal surfaces.<h4>Methods</h4>New Zealand rabbits (<i>n</i> = 10) were distributed in two groups: Group Bioscaffold alone (G1)-decellularized vena cava (1 cm<sup>2</sup>) was implanted, and Group Bioscaffold plus ACSs (G2)-decellularized vena cava (1 cm<sup>2</sup>) containing ASCs were implanted. ASCs we ...[more]

PMID: 36359335

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Dataset Information

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits.

Background

Methods

Results

Conclusion

Publications

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits.

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