Human 3D Airway Tissue Models for Real-Time Microscopy: Visualizing Respiratory Virus Spreading.

ABSTRACT: Our knowledge about respiratory virus spreading is mostly based on monolayer cultures that hardly reflect the complex organization of the airway epithelium. Thus, there is a strong demand for biologically relevant models. One possibility to study virus spreading at the cellular level is real-time imaging. In an attempt to visualize virus spreading under somewhat more physiological conditions, Calu-3 cells and human primary fibroblasts were co-cultured submerged or as air-liquid interface (ALI). An influenza A virus (IAV) replicating well in cell culture, and carrying a red fluorescent protein (RFP) reporter gene was used for real-time imaging. Our three-dimensional (3D) models exhibited important characteristics of native airway epithelium including a basement membrane, tight junctions and

SUBMITTER: Mockel M 

PROVIDER: S-EPMC9688616 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature