Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).

ABSTRACT: Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in a swellable polymer gel to physically expand the sample and isotropically increase resolution in x, y, and z. The maximum resolution increase is limited by the expansion factor of the gel, which is four-fold for the original ExM protocol. Variations on the original ExM method have been reported that allow for greater expansion factors but at the cost of ease of adoption or versatility. Here, we systematically explore the ExM recipe space and present a novel method termed Ten-fold Robust Expansion Microscopy (TREx) that, like the original ExM method, requires no specialized equipment or procedures. We demonstr

SUBMITTER: Damstra HGJ 

PROVIDER: S-EPMC8887890 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature