New Effective Method of Lactococcus Genome Editing Using Guide RNA-Directed Transposition.

ABSTRACT: Lactococcus lactis is an important industrial microorganism and a widely used model object for research in the field of lactic acid bacteria (LAB) biology. The development of new L. lactis and related LAB strains with improved properties, including phage-resistant strains for dairy fermentation, LAB-based vaccines or strains with altered genotypes for research purposes, are hindered by the lack of genome-editing tools that allow for the easy and straightforward incorporation of a significant amount of the novel genetic material, such as large genes or operons, into the chromosomes of these bacteria. We recently employed a suggested system based on the CRISPR-Cas-associated transposon for the editing of the L. lactis genome. After the in-depth redesign of the system, we

SUBMITTER: Pechenov PY 

PROVIDER: S-EPMC9696066 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature