Unknown

Dataset Information

0

Inter-Fighting between Influenza A Virus NS1 and β-TrCP: A Novel Mechanism of Anti-Influenza Virus.


ABSTRACT: Influenza A virus (IAV) prevents innate immune signaling during infection. In our previous study, the production of pro-inflammatory cytokines was associated with Cullin-1 RING ligase (CRL1), which was related to NF-κB activation. However, the underlying mechanism is unclear. Here, an E3 ligase, β-transducin repeat-containing protein (β-TrCP), was significantly downregulated during IAV infection. Co-IP analysis revealed that non-structural 1 protein (NS1) interacts with β-TrCP. With co-transfection, an increase in NS1 expression led to a reduction in β-TrCP expression, affecting the level of IκBα and then resulting in repression of the activation of the NF-κB pathway during IAV infection. In addition, β-TrCP targets the viral NS1 protein and significantly reduces the replication level of influenza virus. Our results provide a novel mechanism for influenza to modulate its immune response during infection, and β-TrCP may be a novel target for influenza virus antagonism.

SUBMITTER: Sun H 

PROVIDER: S-EPMC9699209 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Inter-Fighting between Influenza A Virus NS1 and β-TrCP: A Novel Mechanism of Anti-Influenza Virus.

Sun Haiwei H   Wang Kai K   Yao Wei W   Liu Jingyi J   Lv Lu L   Shi Xinjin X   Chen Hongjun H  

Viruses 20221031 11


Influenza A virus (IAV) prevents innate immune signaling during infection. In our previous study, the production of pro-inflammatory cytokines was associated with Cullin-1 RING ligase (CRL1), which was related to NF-κB activation. However, the underlying mechanism is unclear. Here, an E3 ligase, β-transducin repeat-containing protein (β-TrCP), was significantly downregulated during IAV infection. Co-IP analysis revealed that non-structural 1 protein (NS1) interacts with β-TrCP. With co-transfect  ...[more]

Similar Datasets

| S-EPMC1933316 | biostudies-literature
| S-EPMC6205234 | biostudies-literature
| S-EPMC6289448 | biostudies-literature
| S-EPMC6153301 | biostudies-literature
2012-07-10 | GSE39202 | GEO
| S-EPMC10612106 | biostudies-literature
2012-07-09 | E-GEOD-39202 | biostudies-arrayexpress
| S-EPMC6258958 | biostudies-literature
| S-EPMC3185538 | biostudies-literature
| S-EPMC3993732 | biostudies-literature