CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data.

ABSTRACT:

Background

Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced pluripotent stem cells (hiPSCs), are lacking. Existing methods lack scalability to accommodate high-throughput comparisons between multiple lines, across developmental timepoints, or across pharmacological manipulations.

Results

To address this need we developed CaPTure, a scalable, automated Ca²⁺ imaging analysis pipeline ( https://github.com/LieberInstitute/CaPTure ). CaPTuredetects neurons, classifies and quantifies spontaneous activity, quantifies synchrony metrics, and generates cell- and network-specific metric

SUBMITTER: Tippani M 

PROVIDER: S-EPMC9710137 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature