Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes.

ABSTRACT: Post-transcriptional gene silencing using double-stranded RNA has revolutionized the field of functional genetics, allowing fast and easy disruption of gene function in various organisms. In Drosophila, many transgenic RNAi lines have been generated in large-scale efforts, including the Drosophila Transgenic RNAi Project (TRiP), to facilitate in vivo knockdown of virtually any Drosophila gene with spatial and temporal resolution. The available transgenic RNAi lines represent a fundamental resource for the fly community, providing an unprecedented opportunity to address a vast range of biological questions relevant to basic and biomedical research fields. However, caution should be applied regarding the efficiency and specificity of the RNAi approach. Here, we demonstra

SUBMITTER: Stankovic D 

PROVIDER: S-EPMC9711858 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature