Physiological intron retaining transcripts in the cytoplasm abound during human motor neurogenesis.

ABSTRACT: Intron retention (IR) is now recognized as a dominant splicing event during motor neuron (MN) development; however, the role and regulation of intron-retaining transcripts (IRTs) localized to the cytoplasm remain particularly understudied. Here we show that IR is a physiological process that is spatiotemporally regulated during MN lineage restriction and that IRTs in the cytoplasm are detected in as many as 13% (n = 2297) of the genes expressed during this process. We identify a major class of cytoplasmic IRTs that are not associated with reduced expression of their own genes but instead show a high capacity for RNA-binding protein and miRNA occupancy. Finally, we show that ALS-causing VCP mutations lead to a selective increase in cytoplasmic abundance of this particular clas

SUBMITTER: Petric Howe M 

PROVIDER: S-EPMC9712626 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature