Dataset Information

Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study

ABSTRACT:

Objective

To examine the plasma microbiome for differences between obese individuals with and without osteoarthritis (OA) and its association with serum lipopolysaccharide (LPS).

Design

Blood samples from 70 participants with body mass index (BMI) ≥ 30kg/m2 and age ≥55 years, with (cases) or without (controls) hand plus knee OA, were analyzed for serum LPS and composition of the plasma microbiome. The Dirichlet-multinominal recursive partitioning model (DM-RPart) was applied to microbiome compositional data to test the hypothesis that LPS levels distinguish plasma microbiome, accounting for BMI and age.

Results

No significant differences in alpha diversity, or compositional differences between groups at the genus level, were seen between cases and controls (p = 0.11). β-Diversity was significantly associated with serum LPS levels (p = 0.01). DM-RPart resulted in an optimal tree with 3 divisions: 1) based on age (split at 69 years); 2) those older than 69 were split based on BMI; 3) those with BMI <39 kg/m2 were split based on LPS level (at 65 EU/ml). This resulted in 4 groups (nodes 2, and 5–7). Participants in node 2 were younger and the majority had no or mild OA. Those in nodes 5 and 6 were comparable in age and BMI but node 6 had higher LPS and more severe OA. Individuals in node 7 were older, had higher BMI, and the most severe OA.

Conclusions

Our results suggest a relationship between serum LPS and the plasma microbiome in a subgroup of obese individuals with hand plus knee OA that could reflect differences in intestinal permeability.

SUBMITTER: Arbeeva L

PROVIDER: S-EPMC9718202 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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