Single-cell transcriptomes and runx2b^-/- mutants reveal the genetic signatures of intermuscular bone formation in zebrafish.

ABSTRACT: Intermuscular bones (IBs) are mineralized spicules, present in the myosepta of many, but not all, teleost species. IBs are often small and sharp, and they consequently limit how the fish can be processed; the IBs may cause injury or trauma if lodged in consumers' throats or mouths, and therefore affect the appeal of the fish to many consumers. The development of IBs in teleosts is still not fully understood and the molecular basis of IB development remains to be established. Here, the characteristics of IB tissue are evaluated based on single-cell transcriptomics in wild-type zebrafish. The analysis defined 18 distinct cell types. Differentiation trajectories showed that IBs are derived from tendons and that a core tendon-osteoblast cell lineage is related to IB formation. In particular, t

SUBMITTER: Nie CH 

PROVIDER: S-EPMC9718792 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature