Sfrp4 expression in thyroxine treated calvarial cells.

ABSTRACT:

Aims

Evidence suggests alterations of thyroid hormone levels can disrupt normal bone development. Most data suggest the major targets of thyroid hormones to be the Htra1/Igf1 pathway. Recent discovery by our group suggests involvement of targets WNT pathway, specifically overexpression of antagonist Sfrp4 in the presence of exogenous thyroid hormone.

Main methods

Here we aimed to model these interactions in vitro using primary and isotype cell lines to determine if thyroid hormone drives increased Sfrp4 expression in cells relevant to craniofacial development. Transcriptional profiling, bioinformatics interrogation, protein and function analyses were used.

Key findings

Affymetrix transcriptional profiling found Sfrp4 overexpression in primary cranial suture derived ce

SUBMITTER: Durham EL 

PROVIDER: S-EPMC9719041 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature