The intrarenal landscape of T cell receptor repertoire in clear cell renal cell cancer.

ABSTRACT:

Background

Clear cell renal cell cancer (ccRCC) is accompanied by T-cell infiltration. In this study, we sought to determine the difference in T-cell infiltration and the T-cell receptor (TCR) immune repertoire between ccRCC and peritumour tissue.

Methods

T-cell infiltration was examined using immunohistochemistry (IHC) and haematoxylin and eosin (HE) staining. The chi-squared test and Pearson correlation analysis were applied to evaluate the relationship between clinical traits and CD3, CD4, and CD8 expression. Immune repertoire sequencing (IR-Seq) was used to describe the profile of the TCR repertoire.

Results

The adjacent tissue showed increased expression of CD3, CD4 and CD8 compared with ccRCC tissue (P_CD3 = 0.033; P_CD4 = 0.014; P_CD8

SUBMITTER: Zhang W 

PROVIDER: S-EPMC9719196 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature