In Silico and In Vitro Investigation of Anti Helicobacter Activity of Selected Phytochemicals.

ABSTRACT:

Introduction

Helicobacter pylori is Gram-negative helical bacteria that inhibit stomach mucosal lining and establish infection. Urease enzyme was confirmed to be pivotal target in which its suppression will prompt bacteria treatment and eradication.

Methods

Series of naturally bioactive compounds were selected based on ethnobotanical and molecular modeling techniques with potential urease inhibitory effect. The selected phytochemical compounds were in-silico and in-vitro assayed against urease enzyme, minimal inhibitory concentrations (MIC) and a synergistic effect was studied and cultured specifically for H. pylori.

Results

Terpineol was considered as the most active compound with an IC₅₀ of 1.443 μg/ml (R ² =

SUBMITTER: Al Tawalbeh D 

PROVIDER: S-EPMC9728063 | biostudies-literature | 2022 Jul-Sep

REPOSITORIES: biostudies-literature