Dataset Information

In Silico and In Vitro Investigation of Anti Helicobacter Activity of Selected Phytochemicals.

ABSTRACT:

Introduction

Helicobacter pylori is Gram-negative helical bacteria that inhibit stomach mucosal lining and establish infection. Urease enzyme was confirmed to be pivotal target in which its suppression will prompt bacteria treatment and eradication.

Methods

Series of naturally bioactive compounds were selected based on ethnobotanical and molecular modeling techniques with potential urease inhibitory effect. The selected phytochemical compounds were in-silico and in-vitro assayed against urease enzyme, minimal inhibitory concentrations (MIC) and a synergistic effect was studied and cultured specifically for H. pylori.

Results

Terpineol was considered as the most active compound with an IC₅₀ of 1.443 μg/ml (R ² = 0.9374). The synergistic effect of terpineol and metronidazole indicated a possible additive effect (fractional inhibitory concentration result is 0.78) with improvement of MIC results for both terpineol and metronidazole.

Conclusion

This study suggests that terpineol is best to be considered as a lead compound for H. pylori infection treatment and could be a potent inhibitor when combined with metronidazole targeting urease enzyme.

SUBMITTER: Al Tawalbeh D

PROVIDER: S-EPMC9728063 | biostudies-literature | 2022 Jul-Sep

REPOSITORIES: biostudies-literature

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Publications

<i>In Silico</i> and <i>In Vitro</i> Investigation of Anti <i>Helicobacter</i> Activity of Selected Phytochemicals.

Al Tawalbeh Deniz D Aburjai Talal T Al Balas Qosay Q Al Samydai Ali A

Journal of pharmacy & bioallied sciences 20220701 3

<h4>Introduction</h4><i>Helicobacter pylori</i> is Gram-negative helical bacteria that inhibit stomach mucosal lining and establish infection. Urease enzyme was confirmed to be pivotal target in which its suppression will prompt bacteria treatment and eradication.<h4>Methods</h4>Series of naturally bioactive compounds were selected based on ethnobotanical and molecular modeling techniques with potential urease inhibitory effect. The selected phytochemical compounds were <i>in-silico</i> and <i>i ...[more]

PMID: 36506725

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