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Protocol for assessing phagocytosis activity in cultured primary murine microglia.

ABSTRACT: In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe treatment with lipopolysaccharide (LPS) to induce phagocytosis of fluorescent beads, followed by quantitative analysis using fluorescent imaging and Fiji - ImageJ software. For complete details on the use and execution of this protocol, please refer to Parrott et al.¹.

SUBMITTER: Layman E

PROVIDER: S-EPMC9730218 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Protocol for assessing phagocytosis activity in cultured primary murine microglia.

Layman Elsie E Parrott Jennifer Michelle JM Lee Hye Young HY

STAR protocols 20221206 4

In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe treatment with lipopolysaccharide (LPS) to induce phagocytosis of fluorescent beads, followed by quantitative analysis using fluorescent imaging and Fiji - ImageJ software. For complete details on the u ...[more]

PMID: 36595893

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