Highly efficient fermentation of 5-keto-D-fructose with Gluconobacter oxydans at different scales.

ABSTRACT:

Background

The global market for sweeteners is increasing, and the food industry is constantly looking for new low-caloric sweeteners. The natural sweetener 5-keto-D-fructose is one such candidate. 5-Keto-D-fructose has a similar sweet taste quality as fructose. Developing a highly efficient 5-keto-D-fructose production process is key to being competitive with established sweeteners. Hence, the 5-keto-D-fructose production process was optimised regarding titre, yield, and productivity.

Results

For production of 5-keto-D-fructose with G. oxydans 621H ΔhsdR pBBR1-p264-fdhSCL-ST an extended-batch fermentation was conducted. During fructose feeding, a decreasing respiratory activity occurred, despite sufficient carbon supply. Oxygen and second substrate limitation could be exclu

SUBMITTER: Battling S 

PROVIDER: S-EPMC9741787 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature