IL-4-Induced Quiescence of Resting Naive B Cells Is Disrupted in Systemic Lupus Erythematosus.

ABSTRACT: Activated naive (aNAV) B cells have been shown to be the precursor of the CD11c⁺T-bet⁺ IgD^-CD27^- double-negative (DN)2 or atypical memory (aMEM) B cells in systemic lupus erythematosus (SLE). To determine factors that maintain resting naive (rNAV) B cells, the transcriptomic program in naive (IGHD⁺IGHM ⁺) B cells in human healthy control subjects (HC) and subjects with SLE was analyzed by single-cell RNA-sequencing analysis. In HC, naive B cells expressed IL-4 pathway genes, whereas in SLE, naive B cells expressed type I IFN-stimulated genes (ISGs). In HC, aNAV B cells exhibited upregulation of the gene signature of germinal center and classical memory (cMEM) B cells. In contrast, in SLE, aNAV B cells expressed signat

SUBMITTER: Gao M 

PROVIDER: S-EPMC9741951 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature