Conformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis.

ABSTRACT:

Background

In endothelial cells, phospholipase C (PLC) β1-activated Ca²⁺ is a crucial second messenger for the signaling pathways governing angiogenesis. PLCβ1 is inactivated by complexing with an intracellular protein called translin-associated factor X (TRAX). This study demonstrates specific interactions between Globo H ceramide (GHCer) and TRAX, which highlight a new angiogenic control through PLCβ1 activation.

Methods

Globo-series glycosphingolipids (GSLs), including GHCer and stage-specific embryonic antigen-3 ceramide (SSEA3Cer), were analyzed using enzyme-linked immunosorbent assay (ELISA) and Biacore for their binding with TRAX. Angiogenic activities of GSLs in human umbilical vein endothelial cells (HUVECs) were evaluated. Molecular dynamics (MD) simulat

SUBMITTER: Wang SH 

PROVIDER: S-EPMC9753400 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature