Dataset Information

The preservable effects of ultrasound-assisted alginate oligosaccharide soaking on cooked crayfish subjected to Freeze-Thaw cycles.

ABSTRACT: To improve the quality of cooked and frozen crayfish after repeated freeze-thaw cycles, the effects of alginate oligosaccharide (1 %, w/v) with ultrasound-assisted (40 W, 3 min) soaking (AUS) on the physicochemical properties were investigated. The AUS samples improved water-holding capacity with 19.47 % higher than the untreated samples. Low-field nuclear magnetic resonance confirmed that mobile water (T₂₂) in the samples after 5 times of freeze-thaw cycles was reduced by 13.02 % and 29.34 % with AUS and without treatment, correspondingly; and with AUS and without treatment, average size of the ice crystals was around 90.26 μm² and 113.73 μm², and average diameter of the ice crystals was 5.83 μm and 8.14 μm, respectively; furthermore, it enhanced the solubility and zeta potential, lowered the surface hydrophobicity, reduced the particle size, and maintained the secondary and tertiary structures of myofibrillar protein (MP) after repeated freeze-thawing. Gel electrophoresis revealed that the AUS treatment mitigated the denaturation of MPs. Scanning electron microscopy revealed that the AUS treatment preserved the structure of the tissue. These findings demonstrated that the AUS treatment could enhance the water retention and physicochemical properties of protein within aquatic meat products during temperature fluctuations..

SUBMITTER: Han J

PROVIDER: S-EPMC9758566 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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The preservable effects of ultrasound-assisted alginate oligosaccharide soaking on cooked crayfish subjected to Freeze-Thaw cycles.

Han Jiping J Sun Yingjie Y Zhang Tao T Wang Cheng C Xiong Lingming L Ma Yanhong Y Zhu Yongzhi Y Gao Ruichang R Wang Lin L Jiang Ning N

Ultrasonics sonochemistry 20221208

To improve the quality of cooked and frozen crayfish after repeated freeze-thaw cycles, the effects of alginate oligosaccharide (1 %, w/v) with ultrasound-assisted (40 W, 3 min) soaking (AUS) on the physicochemical properties were investigated. The AUS samples improved water-holding capacity with 19.47 % higher than the untreated samples. Low-field nuclear magnetic resonance confirmed that mobile water (T<sub>22</sub>) in the samples after 5 times of freeze-thaw cycles was reduced by 13.02 % and ...[more]

PMID: 36502681

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Project description:The Ili River Valley in Xinjiang, China, is a typical seasonal frozen area where loess landslide disasters have become increasingly common during the freeze-thaw periods in recent years. This study analyzed the macroscopic mechanical strength and microstructure changes of the Ili loess under different freeze-thaw cycles (FTCs) through the post-freeze-thaw triaxial compression test on the unsaturated soil in laboratory. Apart from the scanning electron microscopy (SEM), and the nuclear magnetic resonance (NMR), the macro-micro correlation analysis and the cluster-principal component analysis were applied for the theoretical discussion. The results indicated that the cohesive force of the loess exhibits an initial decreases, followed by the increases, and eventually keep stable after various FTCs, while the internal friction angle showed the opposite developing trend before the final constant. Similar to the strong correlation between the cohesive force and the particle abundance, the internal friction angle is also closely related to the abundance and orientation fractal dimension of the loess particles. However, the principal component analysis results showed that cohesive force strongly correlates with the average maximum pore size and the pore size fractal dimension, for which the internal friction angle most strongly affected by the average maximum particle size. The possible reason is that the extracted principal components represent a class of microscopic parameters with the same or similar change trend, although there may be a certain offset between them. The mechanical deterioration of loess is attributed to the repeated frost heaving force and the migration potential caused by FTCs. The alterations of the microstructure accelerated the deterioration of the macroscopic mechanical properties of the loess, which further widens the understanding of the mechanism behind the deterioration of loess mechanical strength in the Ili River Valley under FTCs, and contributes to the prevention and management of the local landslide disasters.

Project description:Tissue material properties are crucial to understanding their mechanical function, both in healthy and diseased states. However, in certain circumstances logistical limitations can prevent testing on fresh samples necessitating one or more freeze-thaw cycles. To date, the nature and extent to which the material properties of articular cartilage are altered by repetitive freezing have not been explored. Therefore, the aim of this study is to quantify how articular cartilage mechanical properties, measured by nanoindentation, are affected by multiple freeze-thaw cycles. Canine cartilage plugs (n = 11) from medial and lateral femoral condyles were submerged in phosphate buffered saline, stored at 3-5°C and tested using nanoindentation within 12h. Samples were then frozen at -20°C and later thawed at 3-5°C for 3h before material properties were re-tested and samples re-frozen under the same conditions. This process was repeated for all 11 samples over three freeze-thaw cycles. Overall mean and standard deviation of shear storage modulus decreased from 1.76 ± 0.78 to 1.21 ± 0.77MPa (p = 0.91), shear loss modulus from 0.42 ± 0.19 to 0.39 ± 0.17MPa (p=0.70) and elastic modulus from 5.13 ± 2.28 to 3.52 ± 2.24MPa (p = 0.20) between fresh and three freeze-thaw cycles respectively. The loss factor increased from 0.31 ± 0.38 to 0.71 ± 1.40 (p = 0.18) between fresh and three freeze-thaw cycles. Inter-sample variability spanned as much as 10.47MPa across freezing cycles and this high-level of biological variability across samples likely explains why overall mean "whole-joint" trends do not reach statistical significance across the storage conditions tested. As a result multiple freeze-thaw cycles cannot be explicitly or statistically linked to mechanical changes within the cartilage. However, the changes in material properties observed herein may be sufficient in magnitude to impact on a variety of clinical and scientific studies of cartilage, and should be considered when planning experimental protocols.

Dataset Information

The preservable effects of ultrasound-assisted alginate oligosaccharide soaking on cooked crayfish subjected to Freeze-Thaw cycles.

Publications

The preservable effects of ultrasound-assisted alginate oligosaccharide soaking on cooked crayfish subjected to Freeze-Thaw cycles.

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