Ultrasensitive PCR-Free detection of whole virus genome by electrochemiluminescence.

ABSTRACT: Detection of nucleic acids is crucial in many medical applications, and in particular for monitoring infectious diseases, as it has become perfectly clear after the pandemic infection of COVID-19. In this context, the development of innovative detection methods based on signal-amplification rather than analyte-amplification represents a significant breakthrough compared to existing PCR-based methodologies, allowing the development of new nucleic acid detection technologies suitable to be integrated in portable and low-cost sensor devices while keeping high sensitivities, thus enabling massive diagnostic screening. In this work, we present a novel molecular sensor for the ultrasensitive PCR-free detection of Hepatitis B Virus (HBV) based on electrochemiluminescence (ECL). Thanks to the comb

SUBMITTER: Nikolaou P 

PROVIDER: S-EPMC9759511 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature