Protocol for production and expression of chimeric bovine-human monoclonal antibodies.
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ABSTRACT:
SUBMITTER: Heydarchi B
PROVIDER: S-EPMC9763940 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
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Protocol for production and expression of chimeric bovine-human monoclonal antibodies.
Heydarchi Behnaz B Purcell Damian F J DFJ
STAR protocols 20221209 4
We describe herein a protocol for production of chimeric bovine-human monoclonal antibodies (mAbs) from vaccinated cows. The genes of HIV-1-specific single B cells are amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned into human expression vectors, and expressed in human cell lines. This protocol provides an efficient step-by-step methodology to produce HIV-1 chimeric mAbs and could be widely adapted for other antigens. For complete details on the use and execution of ...[more]
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