Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera.

ABSTRACT:

Background

Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic research. The protoplast and transient transformation system plays an important role in biological breeding, plant regeneration and somatic cell fusion. The objective of this present study was to develop a highly efficient protocol for isolating and purifying mesophyll protoplasts and transient transformation of C. oleifera. Several critical factors for mesophyll protoplast isolation from C. oleifera, including starting material (leaf age), pretreatment, enzymatic treatment (type of enzyme, concentration and digestion time), osmotic pressure and purification were optimiz

SUBMITTER: Li S 

PROVIDER: S-EPMC9773467 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature