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Protein Supplementation during Mid-Gestation Alters the Amino Acid Patterns, Hepatic Metabolism, and Maternal Skeletal Muscle Turnover of Pregnant Zebu Beef Cows.


ABSTRACT: From 100 to 200 days of gestation, 52 cows carrying male (n = 30) or female (n = 22) fetuses were assigned to CON (basal diet-5.5% of CP, n = 26) or SUP (basal diet + protein supplement [40% CP, 3.5 g/kg BW]-12% of CP, n = 26) treatments. Glucose concentrations decreased at 200 (p ≤ 0.01; CON = 46.9 and SUP = 54.7 mg/dL) and 270 days (p ≤ 0.05; CON = 48.4 and SUP = 53.3 mg/dL) for CON compared to SUP. The same pattern occurred for insulin (p ≤ 0.01). At parturition, the NEFA concentration was greater (p = 0.01, 0.10 vs. 0.08 mmol/L) for CON than for SUP. Total AA increased in SUP (p ≤ 0.03) at mid- and late-gestation compared to CON. At 200 days, CON dams carrying females had less essential AA (p = 0.01) than cows carrying males. The SUP dams had greater expressions of protein synthesis markers, namely eIf4E and GSK3β (p ≤ 0.04), at day 200 and of MuFR1 (protein degradation marker, p ≤ 0.04) at parturition. Supplemented cows had higher hepatic pyruvate carboxylase expressions (p = 0.02). Therefore, PS alleviates the restriction overload on maternal metabolism.

SUBMITTER: Meneses JAM 

PROVIDER: S-EPMC9774392 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Protein Supplementation during Mid-Gestation Alters the Amino Acid Patterns, Hepatic Metabolism, and Maternal Skeletal Muscle Turnover of Pregnant Zebu Beef Cows.

Meneses Javier Andrés Moreno JAM   Nascimento Karolina Batista KB   Galvão Matheus Castilho MC   Ramírez-Zamudio German Darío GD   Gionbelli Tathyane Ramalho Santos TRS   Ladeira Marcio Machado MM   Duarte Marcio de Souza MS   Casagrande Daniel Rume DR   Gionbelli Mateus Pies MP  

Animals : an open access journal from MDPI 20221216 24


From 100 to 200 days of gestation, 52 cows carrying male (<i>n</i> = 30) or female (<i>n</i> = 22) fetuses were assigned to CON (basal diet-5.5% of CP, <i>n</i> = 26) or SUP (basal diet + protein supplement [40% CP, 3.5 g/kg BW]-12% of CP, <i>n</i> = 26) treatments. Glucose concentrations decreased at 200 (<i>p</i> ≤ 0.01; CON = 46.9 and SUP = 54.7 mg/dL) and 270 days (<i>p</i> ≤ 0.05; CON = 48.4 and SUP = 53.3 mg/dL) for CON compared to SUP. The same pattern occurred for insulin (<i>p</i> ≤ 0.0  ...[more]

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