TWIST1 Plays Role in Expression of Stemness State Markers in ESCC.

ABSTRACT:

Background

Stemness markers play critical roles in the maintenance of key properties of embryonic stem cells (ESCs), including the pluripotency, stemness state, and self-renewal capacities, as well as cell fate decision. Some of these features are present in cancer stem cells (CSCs). TWIST1, as a bHLH transcription factor oncogene, is involved in the epithelial-mesenchymal transition (EMT) process in both embryonic and cancer development. Our aim in this study was to investigate the functional correlation between TWIST1 and the involved genes in the process of CSCs self-renewal in human esophageal squamous cell carcinoma (ESCC) line KYSE-30.

Methods

TWIST1 overexpression was enforced in the ESCC KYSE-30 cells using retroviral vector containing the specific pruf

SUBMITTER: Izadpanah MH 

PROVIDER: S-EPMC9777594 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature