Dataset Information

Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing.

ABSTRACT:

Objective

Klebsiella pneumoniae is a common multidrug-resistant pathogen that jeopardizes the health of hospitalized patients. We aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant K. pneumoniae (CRKP) isolates from a hospital in Beijing.

Methods

Twenty-four CRKP clinical isolates were collected within a half-year to investigate antimicrobial resistance and genomic characteristics. Illumina and Nanopore sequencing were performed to assemble and annotate genomes.

Results

All strains were multi-drug resistant. Twenty-two strains carried the bla _KPC-2 gene and two harbored bla _NDM-5. Multilocus sequence type(MLST) analysis identified five sequence types; most isolates belonged to ST11. Three strains were isolated from the same patient; each carried a different plasmid replicon, either IncFII _(pHN7A8), IncX, or IncFIB _(K).

Conclusion

This study furthers the understanding of CRKP antimicrobial resistance genotypes, and may facilitate the control of nosocomial infections caused by antimicrobial-resistant pathogens.

SUBMITTER: Ni Q

PROVIDER: S-EPMC9784392 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Genetic and Phenotypic Characteristics of Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates from a Tertiary Hospital in Beijing.

Ni Qin Q Yao Xingwei X Li Jinhui J Ma Jinghan J Wang Kaiying K Liu Xiong X Li Peihan P Yang Lang L Li Peng P Li Shenlong S

Infection and drug resistance 20221219

<h4>Objective</h4><i>Klebsiella pneumoniae</i> is a common multidrug-resistant pathogen that jeopardizes the health of hospitalized patients. We aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant <i>K. pneumoniae</i> (CRKP) isolates from a hospital in Beijing.<h4>Methods</h4>Twenty-four CRKP clinical isolates were collected within a half-year to investigate antimicrobial resistance and genomic characteristics. Illumina and Nanopore sequencing were performed to as ...[more]

PMID: 36570713

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