Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.

ABSTRACT: A stunning example of synaptic diversity is the postsynaptic target cell-type-dependent difference in synaptic efficacy in cortical networks. Here, we show that CA1 pyramidal cell (PC) to fast spiking interneuron (FSIN) connections have 10-fold larger release probability (P_v) than those on oriens lacunosum-moleculare (O-LM) interneurons. Freeze-fracture immunolabeling revealed that different nano-topologies and coupling distances between Ca²⁺ channels and release sites (RSs) are not responsible for the distinct P_v. Although [Ca²⁺] transients are 40% larger in FSINs innervating boutons, when [Ca²⁺] entry is matched in the two bouton populations, EPSCs in O-LM cells are still 7-fold smaller. However, application of a phorbol ester analog

SUBMITTER: Aldahabi M 

PROVIDER: S-EPMC9796815 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature