Detergent exposure induces epithelial barrier dysfunction and eosinophilic inflammation in the esophagus.

ABSTRACT:

Background

Eosinophilic esophagitis (EoE) is a chronic allergic disease associated with type 2 inflammation and epithelial barrier dysfunction. The etiology is unknown, however, genetic heritability studies suggest environmental factors play a key role in pathogenesis. Detergents, such as sodium dodecyl sulfate (SDS), are common ingredients in household products such as dish soap and toothpaste. We hypothesized detergent exposure decreases epithelial barrier function and induces esophageal inflammation.

Methods

Immortalized esophageal epithelial cells (EPC2) were cultured in air-liquid interface (ALI) and exposed to SDS. Barrier function/activity was assessed by transepithelial electrical resistance (TEER), FITC-dextran flux, and RT-PCR. Additionally, SDS-treated mouse esoph

SUBMITTER: Doyle AD 

PROVIDER: S-EPMC9797443 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature