Pulmonary EV miRNA profiles identify disease and distinct inflammatory endotypes in COPD.

ABSTRACT:

Introduction

Chronic obstructive pulmonary disease (COPD) is a heterogeneous condition without effective disease modifying therapies. Identification of novel inflammatory endotype markers such as extracellular vesicles (EVs), which are important intercellular messengers carrying microRNA (miRNA), may enable earlier diagnosis and disease stratification for a targeted treatment approach. Our aim was to identify differentially expressed EV miRNA in the lungs of COPD patients compared with healthy ex-smokers and determine whether they can help define inflammatory COPD endotypes.

Methods

EV miRNA were isolated and sequenced from ex-smoking COPD patients and healthy ex-smoker bronchoalveolar lavage fluid. Results were validated with RT-qPCR and compared to differential inflammator

SUBMITTER: Burke H 

PROVIDER: S-EPMC9797812 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature