Dataset Information

Antimicrobial Activity of Graphene Oxide Contributes to Alteration of Key Stress-Related and Membrane Bound Proteins.

ABSTRACT:

Introduction

Antibacterial activity of graphene oxide (GO) has been extensively studied, wherein penetration of the bacterial cell membrane and oxidative stress are considered to play a major role in the bactericidal activity of GO. However, the specific mechanism responsible for the antibacterial activity of GO remains largely unknown. Hence, the goal of this study was to explore the mode of action of GO, via an in-depth proteomic analysis of the targeted bacteria.

Methods

Staphylococcus aureus was grown in the presence of GO and samples were collected at different growth phases to examine the cell viability and to analyze the changes in protein expression. Antimicrobial efficiency of GO was tested by assessing bacterial viability, live/dead staining and scanning electron microscopy. The intracellular reactive oxygen species (ROS) induced by GO treatment were examined by fluorescence microscopy. Label-free quantitative proteomics analysis was performed to examine the differentially regulated proteins in S. aureus after GO treatment.

Results

GO treatment was observed to reduce S. aureus viability, from 50 ± 17% after 4 h, to 93 ± 2% after 24 h. The live/dead staining confirmed this progressive antimicrobial effect of GO. SEM images revealed the wrapping of bacterial cells and their morphological disruption by means of pore formation due to GO insertion. GO treatment was observed to generate intracellular ROS, correlating to the loss of cell viability. The proteomics analysis revealed alteration in the expression of cell membrane, oxidative stress response, general stress response, and virulence-associated proteins in GO-treated bacterial cells. The time-dependent bactericidal activity of GO correlated with a higher number of differentially regulated proteins involved in the above.-mentioned processes.

Conclusion

The obtained results suggest that the time-dependent bactericidal effect of GO is attributed to its wrapping/trapping ability, ROS production and due to physical disruption of the cell membrane.

SUBMITTER: Ravikumar V

PROVIDER: S-EPMC9805717 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Antimicrobial Activity of Graphene Oxide Contributes to Alteration of Key Stress-Related and Membrane Bound Proteins.

Ravikumar Vaishnavi V Mijakovic Ivan I Pandit Santosh S

International journal of nanomedicine 20221228

<h4>Introduction</h4>Antibacterial activity of graphene oxide (GO) has been extensively studied, wherein penetration of the bacterial cell membrane and oxidative stress are considered to play a major role in the bactericidal activity of GO. However, the specific mechanism responsible for the antibacterial activity of GO remains largely unknown. Hence, the goal of this study was to explore the mode of action of GO, via an in-depth proteomic analysis of the targeted bacteria.<h4>Methods</h4><i>Sta ...[more]

PMID: 36597432

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Project description:Skin wound healing is a complicated and lengthy process, which is influenced by multiple factors and need a suitable cellular micro-environment. For skin wound, wound dressings remain a cornerstone of dermatologic therapy at present. The dressing material can create an effective protective environment for the wound, and the interactions between the dressing and the wound has a great impact on the wound healing efficiency. An ideal wound dressing materials should have good biocompatibility, moisturizing property, antibacterial property and mechanical strength, and can effectively prevent wound infection and promote wound healing. In this study, in order to design wound dressing materials endowed with excellent antibacterial and tissue repair properties, we attempted to load antimicrobial peptides onto dopmine-modified graphene oxide (PDA@GO) using lysozyme (ly) as a model drug. Then, functionalized GO was used to the surface modification of arginine-modified chitosan (CS-Arg) membrane. To evaluate the potential of the prepared nanocomposite membrane in wound dressing application, the surface morphology, hydrophilic, mechanical properties, antimicrobial activity, and cytocompatibility of the resulting nanocomposite membrane were analyzed. The results revealed that prepared nanocomposite membrane exhibited excellent hydrophilic, mechanical strength and antimicrobial activity, which can effectively promote cell growth and adhesion. In particular, using PDA@GO as drug carrier can effectively maintain the activity of antimicrobial peptides, and can maximize the antibacterial properties of the nanocomposite membrane. Finally, we used rat full-thickness wound models to observe wound healing, and the surface interactions between the prepared nanocomposite membrane and the wound. The results indicated that nanocomposite membrane can obviously accelerated wound closure, and the wounds showed reduced inflammation, improved angiogenesis and accelerated re-epithelialization. Therefore, incorporation of antimicrobial peptides-functionalize graphene oxide (ly-PDA@GO) into CS-Arg membrane was a viable strategy for fabricating excellent wound dressing. Together, this study not only prepared a wound dressing with excellent tissue repair ability, but also provided a novel idea for the development of graphene oxide-based antibacterial dressing.

Project description:The rapid increase in the prevalence of antibiotic-resistant bacterial strains poses a global health risk. In this scenario, alternative strategies are needed to combat the alarming rise in multidrug-resistant bacterial populations. For example, metal-incorporated graphene derivatives have emerged as model nanomaterials owing to their intrinsic antibacterial activity together with their biocompatibility. Interestingly, photon-activated phthalocyanine sensitizers have also shown promising physiochemical biocidal effects against pathogenic bacteria populations when conjugated with diverse nanomaterials. Herein, we report the facile synthesis of graphene oxide incorporated zinc phthalocyanine (ZnPc-GO) nanohybrids showing bactericidal activity against Gram-negative Escherichia coli (E. coli) cells, in the absence of any photo-excitation. The ZnPc-GO hybrid nanomaterials were synthesized by the in situ deposition of GO flakes on ZnPc-coated indium tin oxide (ITO) substrates. Two types of morphologically different ZnPc molecules, potato-chip-like α-phase ZnPc, namely ZnPc(A), and nanorod-like β-phase ZnPc(B), were used for the synthesis of the ZnPc(A/B)-GO nanocomposites. The interactions of GO with the underlying ZnPc(A/B) entities in the ZnPc-GO systems were investigated using multiple characterization techniques. It was observed that the GO flakes in the ZnPc(B)-GO nanocomposite possess stronger π-π interactions and thus show a more efficient electron transfer mechanism when compared with the ZnPc(A) counterpart. Furthermore, the E. coli bacterial cells with an electronegative surface demonstrated a profound adherence to the electron-withdrawing ZnPc(B)-GO surface. The death kinetics of bacteria with ZnPc(B)-GO were further investigated using surface potential mapping and Kelvin probe force microscopy (KPFM) analysis. Upon direct contact with ZnPc(B)-GO, the adhered bacterial cells showed outer cell deformation and membrane protein leakage, induced by a proposed charge-transfer mechanism between negatively charged cells and the electron-withdrawing ZnPc(B)-GO surface. These new findings may provide insights into the design of potential ZnPc-GO-based novel antimicrobial nanomaterials or surface coatings.

Dataset Information

Antimicrobial Activity of Graphene Oxide Contributes to Alteration of Key Stress-Related and Membrane Bound Proteins.

Introduction

Methods

Results

Conclusion

Publications

Antimicrobial Activity of Graphene Oxide Contributes to Alteration of Key Stress-Related and Membrane Bound Proteins.

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