Higher Temperature Porous Graphitic Carbon Separations Differentially Impact Distinct Glycopeptide Classes.

ABSTRACT: Mass spectrometry-based discovery glycoproteomics is highly dependent on the use of chromatography paradigms amenable to analyte retention and separation. When compared against established stationary phases such as reversed-phase and hydrophilic interaction liquid chromatography, reports utilizing porous graphitic carbon have detailed its numerous advantages. Recent efforts have highlighted the utility in porous graphitic carbon in high-throughput glycoproteomics, principally through enhanced profiling depth and liquid-phase resolution at higher column temperatures. However, increasing column temperature has been shown to impart disparaging effects in glycopeptide identification. Herein we further elucidate this trend, describing qualitative and semiquantitative effects of increased column

SUBMITTER: Delafield DG 

PROVIDER: S-EPMC9812930 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature