The Male-Biased Expression of miR-2954 Is Involved in the Male Pathway of Chicken Sex Differentiation.

ABSTRACT: Many expression data showed miRNAs have a potential function on regulating gonadal differentiation in animals, but their function is rarely studied in vivo, especially in chickens. Using the comprehensive expression profiles analysis, the specific male-biased miR-2954, which is significantly higher expressed in male embryos and gonads at all detected stages, was firstly screened during the early stages of chicken embryogenesis and gonadogenesis. In sex-reversed female gonads treated with aromatase inhibitors, the expression of miR-2954 was increased, which was consistent with the up-regulation of DMRT1 and SOX9. The injection of vivo-morpholino of miR-2954 significantly inhibited the expression of miR-2954 in chicken embryos, and the down-regulation of miR-2954 decreased the

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC9818168 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature