A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX Interactomes.

ABSTRACT: Biological pathways rely on the formation of intricate protein interaction networks called interactomes. Getting a comprehensive map of interactomes implies the development of tools that allow one to capture transient and low-affinity protein-protein interactions (PPIs) in live conditions. Here we presented an experimental strategy: the Cell-PCA (cell-based protein complementation assay), which was based on bimolecular fluorescence complementation (BiFC) for ORFeome-wide screening of proteins that interact with different bait proteins in the same live cell context, by combining high-throughput sequencing method. The specificity and sensitivity of the Cell-PCA was established by using a wild-type and a single-amino-acid-mutated HOXA9 protein, and the approach was subsequently applied to sev

SUBMITTER: Jia Y 

PROVIDER: S-EPMC9818449 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature